UKIP Leader Nigel Farage urged all Party members and supporters to add their names to the ePetition as soon as possible.
He said: "This is a real chance to force the Government into a proper, Parlimentary debate on an issue that affects everyone in the UK, every single day of their lives but on which they have been denied a say so far.
"So we back the Daily Express call for an EU referendum and I hope every UKIP member, their families, friends and work colleagues will add their names to the ePetition. Use all the tools at your disposal to spread the word. Put it on your Facebook page, re-tweet on Twitter, whatever it takes, spread the word.
"The Government has said it will consider any issue for Parliamentary debate if 100,000 people put their names to it. With the weight of feeling about the EU in this country, particularly over the economic bailouts, we could easily achieve 10 times that number!
"This is also a chance for UKIP to call on support for the petition from across the board and put Nation before Party. At last, we could be given a say on the one big question: In or out?